capturem his tagged purification miniprep kit (TaKaRa)
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capturem his tagged purification miniprep kit
Capturem His Tagged Purification Miniprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capturem+his+tagged+purification+miniprep+kit/Capturem+His-Tagged+Purification+Miniprep+Kit/pm42043053-263-13-19
Average 99 stars, based on 70 article reviews
Capturem His Tagged Purification Miniprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/capturem+his+tagged+purification+miniprep+kit/Capturem+His-Tagged+Purification+Miniprep+Kit/pm42043053-263-13-19
Average 99 stars, based on 70 article reviews
capturem his tagged purification miniprep kit - by Bioz Stars,
2026-09
99/100 stars
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Recombinant:Article Title: Development and Clinical Evaluation of a Mpox Antigen-detecting Rapid Diagnostic Test. Article Snippet: 2.2 Antigen preparation Jo ur n l P re -p ro of Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Article Title: Genome-wide analysis of AP2/ERF family in Akebia trifoliata and characterization of an AtrERF001 gene regulate fruit ripening Article Snippet: The full-length coding sequence of AtrERF001 was cloned and inserted into the pET-32A vector and transformed into Escherichia coli strain BL21 (DE3) (Novagen, Denmark). .. Protein expression was induced using 0.2 mM isopropyl b-D-1-thiogalactopyranoside (IPTG) at 16°C for 20 h. The recombinant protein was purified with a Article Title: Development and clinical evaluation of a monkeypox antigen-detecting rapid diagnostic test Article Snippet: Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Purification:Article Title: Development and Clinical Evaluation of a Mpox Antigen-detecting Rapid Diagnostic Test. Article Snippet: 2.2 Antigen preparation Jo ur n l P re -p ro of Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Article Title: Purification and Characterization of His-Tagged Recombinant Bacteroides fragilis Toxin-2 Variants In Vitro and In Vivo. Article Snippet: His-rNTBF and His-rETBF strains were cultured anaerobically in BHIS broth supplemented with clindamycin 6 μg/mL and gentamicin 50 μg/mL at 37 ◦C for 48 h. Culture supernatants were harvested by centrifugation at 14,000× g for 5 min at 4 ◦C and filtered through 0.45 μm Minisart® Filter Unit (#S6555-FMOSK, Sartorius, Goettingen, Germany) to remove residual bacterial cells. .. For small-scale purification, filtered supernatants (up to 10 mL) were processed using the Article Title: Genome-wide analysis of AP2/ERF family in Akebia trifoliata and characterization of an AtrERF001 gene regulate fruit ripening Article Snippet: The full-length coding sequence of AtrERF001 was cloned and inserted into the pET-32A vector and transformed into Escherichia coli strain BL21 (DE3) (Novagen, Denmark). .. Protein expression was induced using 0.2 mM isopropyl b-D-1-thiogalactopyranoside (IPTG) at 16°C for 20 h. The recombinant protein was purified with a Article Title: Purification and Characterization of His-Tagged Recombinant Bacteroides fragilis Toxin-2 Variants In Vitro and In Vivo Article Snippet: His-rNTBF and His-rETBF strains were cultured anaerobically in BHIS broth supplemented with clindamycin 6 μg/mL and gentamicin 50 μg/mL at 37 °C for 48 h. Culture supernatants were harvested by centrifugation at 14,000× g for 5 min at 4 °C and filtered through 0.45 μm Minisart ® Filter Unit (#S6555-FMOSK, Sartorius, Goettingen, Germany) to remove residual bacterial cells. .. For small-scale purification, filtered supernatants (up to 10 mL) were processed using the Article Title: Development and clinical evaluation of a monkeypox antigen-detecting rapid diagnostic test Article Snippet: Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Article Title: Mushrooms Do Produce Flavonoids: Metabolite Profiling and Transcriptome Analysis of Flavonoid Synthesis in the Medicinal Mushroom Sanghuangporus baumii Article Snippet: The PAL open reading frame was incorporated into the pET-32a vector (TaKaRa, Beijing, China) and transferred into Escherichia coli (TaKaRa, Beijing, China) to express the recombinant protein ( PAL -B, PAL -H, ). .. Protein expression was triggered by the addition of isopropyl-β-D-thiogalactoside (IPTG, TIANGEN, Beijing, China) to an ultimate 1 mmol/L concentration and keeping the temperature at 18 °C for 10 h. The Article Title: Engineering in vitro-assembled beak and feather disease virus-like particles loaded with biomolecules. Article Snippet: Beak and feather disease virus (BFDV) is a member of the Circoviridae family and causes psittacine beak and feather diseases (PBFD) in birds.. Abnormal feathers, brittle claws and beaks, and susceptibility to secondary infections are typical sympotoms of the BFDV-infected birds.. The BFDV virion possesses a single-stranded DNA genome encapsidated within a 17− 22 nm non-enveloped icosahedral virions. Article Title: E3 ligase Praja1 mediates ubiquitination and degradation of microtubule-associated protein tau. Article Snippet: .. A pull-down assay was performed using a Affinity Chromatography:Article Title: Development and Clinical Evaluation of a Mpox Antigen-detecting Rapid Diagnostic Test. Article Snippet: 2.2 Antigen preparation Jo ur n l P re -p ro of Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Article Title: Development and clinical evaluation of a monkeypox antigen-detecting rapid diagnostic test Article Snippet: Chemically synthesized MPXV A5L and VACA A4L cDNA were inserted into the pCold ProS2 vector (Takara Bio, Japan) and transformed into E. coli Tuner (DE3) pLacI (Novagen). .. Recombinant proteins were purified from the soluble fraction of the E. coli lysate via Ni-chelate affinity chromatography ( Expressing:Article Title: Genome-wide analysis of AP2/ERF family in Akebia trifoliata and characterization of an AtrERF001 gene regulate fruit ripening Article Snippet: The full-length coding sequence of AtrERF001 was cloned and inserted into the pET-32A vector and transformed into Escherichia coli strain BL21 (DE3) (Novagen, Denmark). .. Protein expression was induced using 0.2 mM isopropyl b-D-1-thiogalactopyranoside (IPTG) at 16°C for 20 h. The recombinant protein was purified with a Article Title: Mushrooms Do Produce Flavonoids: Metabolite Profiling and Transcriptome Analysis of Flavonoid Synthesis in the Medicinal Mushroom Sanghuangporus baumii Article Snippet: The PAL open reading frame was incorporated into the pET-32a vector (TaKaRa, Beijing, China) and transferred into Escherichia coli (TaKaRa, Beijing, China) to express the recombinant protein ( PAL -B, PAL -H, ). .. Protein expression was triggered by the addition of isopropyl-β-D-thiogalactoside (IPTG, TIANGEN, Beijing, China) to an ultimate 1 mmol/L concentration and keeping the temperature at 18 °C for 10 h. The Concentration Assay:Article Title: Mushrooms Do Produce Flavonoids: Metabolite Profiling and Transcriptome Analysis of Flavonoid Synthesis in the Medicinal Mushroom Sanghuangporus baumii Article Snippet: The PAL open reading frame was incorporated into the pET-32a vector (TaKaRa, Beijing, China) and transferred into Escherichia coli (TaKaRa, Beijing, China) to express the recombinant protein ( PAL -B, PAL -H, ). .. Protein expression was triggered by the addition of isopropyl-β-D-thiogalactoside (IPTG, TIANGEN, Beijing, China) to an ultimate 1 mmol/L concentration and keeping the temperature at 18 °C for 10 h. The Pull Down Assay:Article Title: E3 ligase Praja1 mediates ubiquitination and degradation of microtubule-associated protein tau. Article Snippet: .. A pull-down assay was performed using a |